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Assay Development

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Assay Development

 

We offer custom assay development for a wide range of project needs. Our scientists work diligently to understand your drug discovery project and offer comprehensive strategies towards developing robust assays.

 

Examples of applications include:

    • Protease assays
    • Cell cytotoxicity assay
    • Protein and DNA quantification
    • Molecular Beacons

 

Example Data:


enzymegraphenzyme

 

Figure 1. A full titration series of a compound of interest and model compound were tested against a therapeutic enzymatic target. Selectivity was assessed using enzymes from similar and distant families. Compound X was verified for purity and correct molecular weight using TSQ Quantum Triple Quadrupole LC-MS. Enzymes are assayed in the linear dynamic range so that a titration series of a model compound yields predictable and robust inhibition effects.

 

To get started with Assay Development, contact us so we can discuss your specific project or idea!

 

    Typical assays include:

    • Enzyme activity, drug inhibition and selectivity profiling
    • ELISA development
    • tr-FRET interaction assays
    • In-cell fluorescence
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